BIOCARTE – Biosensors for in situ evaluation of bioavailability of pollutants based on transcriptional regulators a la carte.
This Biosensor allows the quantification of specific molecules in water samples using genetically modified report microorganism.
The core of this technology is the application of high-throughput combinatory genetic technologies to the protein module that sets the specificity of the response to distinct target molecules. This allows the generation and selection of regulator variants able to recognize and to trigger responses to a large variety of chemical species.
The selected proteins will be employed in various biosensor schemes through the coupling of the cognate promoters to genetically formatted lox and gap reporters. This genetic standardisation together with the specialised Software and Hardware development make possible the creation of a innovate biosensor able to quantify optical and metabolic answers generated by the genetically modified microorganisms. This way it is possible to quantify the target molecules.
The BIOCARTE technology, by means of the transcriptional regulators specific to a target molecule, allows modifying microorganism genetically, so that it is possible to add luminescence genes and monitoring the presence of the target molecule in a liquid medium.
This technology, still new to the analytical market, that WOLA is developing can be applied to solve different analytical needs in the real world, not well resolved today, in different areas of industry, biotechnology, biomedicine, environment and public safety.
BIOCARTE developed to quantify the presence -in that occasion- of Xylene in liquid samples. BIOCARTE technology can be customized for the detection of any molecule.
This technology integrates a BOD biosensor, with a molecular affinity biosensor that uses microorganisms sensors that have been genetically modified to produce electromagnetic radiation when there is a presence of the target analyte.
Two cycles of measurement of the presence and quantification of the xylene molecules present in the analysed water.
Blue colour shows the dissolved oxygen concentration during the BOD measurement in the water sample.
In red colour, the luminescence emitted by the sensor cells -which have been genetically modified adding them luciferase- when there is a presence of xylene.
When xylene is present in one of the samples, the luminescence emitted by the luciferase is proportional to the xylene concentration and is quantified by the luminometer.