PRESENTATION

DEVELOP AND IMPLEMENT TECHNOLOGY BIOSENSORSFOR WATER QUALITY AND BIOMEDICINE

Teofilo Díez-Caballero Arnau. GENERAL MANAGER
(Dr. in Medicine/ Dr. Mechanical Engineering)

Fernando Santos Benito. SCIENTIFIC MANAGER
(Dr. in Biochemistry.)

Mª Jesús Aracil. Chemical engineer. R&D Dpt.

Javier Monreal. Engineer in industrial automation and alectronics.

Sergio Montoro. Engineer in Telecommunication

12593 Moncófar.Castellón (Spain).

Tlf. +34 964 579 313

Fax +34 964 579 397

biosensores@biosensores.com

http://www.biosensores.com

INSTRUMENTS DEVELOPED BY BIOSENSORES S.L. :

BOD-MB:	Quantifying contamination biodegradable organic matter in water.
POLITOX:	Quantifying the toxicity comprehensive liquids.
MICROBIO-COUNTER:	Quantifying microoragnismos contaminated liquid.
BIOCARTE- MICROBIAL BIOSENSOR:	Quantifying specific substances in liquid, using genetically modified microorganisms.

Two types of MICROBIAL BIOSENSORS for monitoring BOD continuously. The small size allows the movement of equipment through the use of a vehicle light at different points of application: Studied networks of surface water and wastewater; farms; individualized discharges of polluting enterprises, etc.. The BOD-MB greater size is designed for continuous locations in one place and autonomies team BOD-MB of long duration: continuous monitoring of water input / output WWTP (Waste Water Treatment Plant); control of the spill indrusita a pollutant; maintained surveillance of water quality of a river, an area of moist seawater, and so on.

Interior view of the teams BOD-MB and MINI-BOD-MB. The strategy of our teams anlalítica confers benefits and specifications for applications in the real world that have been recognized by the concerted action "BIOSET " of the European Union:

     * Termostatización of biochemical systems regardless of the temperature outside location (Winter or summer).
     * Renewal of microorganisms sensors at the end of each analysis.
     * Acknowledgement of concentration and metabolic activity of micro-sensors.
     * Double autocalibración in each analytical determination that ensures reliability.
     * Stage automated wash after each analytical determination.
     * Etc..

Acknowledgement of concentration and metabolic activity of micro-sensors, used for determining the BOD in ten water samples from different origins. The blue bars indicate microbial concentration (homogeneous) used by the team BOD-MB in each of the determinations. The red bars indicate the concentration microbial (heterogeneous) of microorganisms present in the samples themselves are responsible for the final determination of BOD-5 in each case. The constancy of microorganisms in the team BOD-MB is one of the characteristics of our technology that makes it more reliable analytical determination of our teams compared to the findings obtained by the standard procedure BOD-5.

The relationship between the data "BOD-MB Microbiosensor" and obtained by the classic method BOD-5, WWTWs in five different sectors in urban and industrial water.
Emphasizing the high correlation between both methods.

Results of monitoring continuously obtained by our team BOD-MB of water quality at Station Control of the Ministry of Environment Spanish in the river Jarama (Madrid, 2000).

For the first time in technological development in continuous international monitoring of water quality was possible in 2000, using one of our prototypes of BOD-MB determine automatically and for an extended period of more than 3 years this parameter in the water of the river Jarama. This allows identify episodes of pollution by BOD allowable levels according to Spanish law (50ppm). This could identify that during January 2000, on certain dates, uncontrolled discharges into the river Jarama, amounted to more than 250ppm values of the BOD.

Details of the registration of automated monitoring of BOD in the river Jarama (Madrid, 2000) done by our team BOD-MB. It can be seen, noting that the data Respirometry values recorded for BOD (A, B, C) are not artifacts analytical errors or operating equipment BOD-MB. So while in the analytical 3 cycles (A, B, C) respirograma of the standard patterns of Reference are held constant (r), respirograma of the sample analysed in the river each (s) (between the two respirogramas standards) is different in each of the cycles.

Data from the PC screen, for the software developed by our company, specifically for monitoring the entry of BOD (red) and output (blue) of a Waste Water Treatment Plant (WWTP). The software also shows the data of Respirometry allow the calculation of BOD in each sample and the date and time at which each sample has been analysed and values of BOD. This new technology allows:

     * Knowing the efficiency in water purification in every moment of laWWTP.
     * Knowing the values point and its evolution over a period of hours, days or months.
     * Feedback these values to optimize efficiency and reduce costs in the exploitation of the WWTP.
     * Ensure the responsible authorities to the efficient functioning of the WWTP at every moment.

APPLICATION OF "BOD-MB BIOSENSORS" IN ACUICULTURE.

[DO]= FUNCTION(BOD, TºC ,PHOTOSINTESYS, ...).

	TECHNOLOGY BIOSENSORES SL	OTHERS TECHNOLOGIES
TIME OF ANALYSIS	35 MINS	5 DAYS (BOD-5)
TIPE OF ANALYSIS	AUTOMATIC	MANUAL
CONTAMINATION REPORTS	YES	NO
COST ANALYSIS	0,8€	48€
CONTROL OF TOXICITY OF WATER	YES	NO
FIABILITY	+++	+
ANALYSIS OF RESULTS	YES	NO

CHARACTERISTICS OF A IDEAL BIOSENSOR
(BIOSET, 2000)

Ruggedness, sensitivity and economic evaluation of biological parameters for the analytical quality of water.
Independence of other environmental parameters such as: toxicity, T º C, etc..
Sensors microbial biological activity with constant.
Autocalibración. Double reference control in each analysis.
Use single microorgansimos for each analysis.
Easy and real-time monitoring to detect alarms quickly.